Amplicon sequencing of size fractionated sea water: microbiota - SRA

SRX26887644: Amplicon sequencing of size fractionated sea water: microbiota
1 ILLUMINA (Illumina MiSeq) run: 29,358 spots, 16.4M bases, 9.8Mb downloads

Design: Samples were collected by niskin at depth, and filtered in series through five nylon mesh, and then a 1.2 and 0.2 micron supore filter. Nylon mesh were themselves backrinsed and the rinse water collected on 1.2 micron durapore filters. At BATS 90m there was also a sinking speed fractionation. Filters were flash frozen. At Horn Point Laboratory, samples were extracted with 10% SDS buffer with alternating hot and bead beating cycles, with two phenol chloroform wash and one cloroform wash. Samples were quantified, diluted, and a known volume of spike was added to each sample (see sample data for amounts). Samples were shipped to MRDNA for library prep and sequencing with 515FY-926R primers on an Illumina MiSeq.

Submitted by: University of Maryland Center for Environmental Science

Study: EPR_Size_Fractionated_Microbiota

PRJNA1191024 • SRP547789 • All experiments • All runs

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Size fractionated amplicon sequence samples from the water column collected on a cruise to the East Pacific Rise in April 2019.

Sample: EPR 316 m 1.2 um

SAMN45067829 • SRS23368030 • All experiments • All runs

Organism: marine metagenome

Library:

Name: 1G3.12PA12

Instrument: Illumina MiSeq

Strategy: AMPLICON